Multivariate analysis using PCA of the parameters in tables 1 and 2 (with BMI and age excluded) using two significant principal components based on eigenvalue criteria (>2).

<p>A. Females with ALS overlapped with female controls in the measured parameters. (t <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113619#pone.0113619-Andersen1" target="_blank">[1]</a>, 34% of variation vs. t <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113619#pone.0113619-Desport1" target="_blank">[2]</a>, 20% of variation; R2 = 0.67; Q2 = .25). B) The male ALS group was shifted from the male controls in the measured parameters. (t <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113619#pone.0113619-Andersen1" target="_blank">[1]</a>, 36% of variation vs. t <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113619#pone.0113619-Desport1" target="_blank">[2]</a>, 15% of variation; R2 = 0.51; Q2 = 0.22).</p

Female subjects Average values ± standard deviation for parameters measured for female controls and ALS patients, fold change (ALS/controls), and p-values for females.

<p>Female subjects Average values ± standard deviation for parameters measured for female controls and ALS patients, fold change (ALS/controls), and p-values for females.</p

Variability in the measured parameters in one female patient with sporadic pseudo bulbar paresis, the samples were collected over 11 months with one time-point in duplicate.

<p>Variability in the measured parameters in one female patient with sporadic pseudo bulbar paresis, the samples were collected over 11 months with one time-point in duplicate.</p

OPLS model predicting survival from the time of sampling for diet-matched ALS cases (p = 0.003).

<p>A. Survival predicted by model plotted against time of survival from sampling. B. Long survival from sampling correlated with higher levels of LDL cholesterol, total cholesterol, coenzyme Q, and VLDL cholesterol.</p

Male subjects Average values ± standard deviation for parameters measured for male controls, ALS patients, fold change (ALS/controls), and p-values for males.

<p>Male subjects Average values ± standard deviation for parameters measured for male controls, ALS patients, fold change (ALS/controls), and p-values for males.</p

Correlation to survival Pearson's correlation for the parameters plotted against survival from time of sampling for all subjects and Spearman's correlation for the parameters plotted against survival from time of sampling for females and males.

<p>Correlation to survival Pearson's correlation for the parameters plotted against survival from time of sampling for all subjects and Spearman's correlation for the parameters plotted against survival from time of sampling for females and males.</p

Correlation between HDL triglycerides and time of survival in female patients (A); HDL triglycerides and time of survival in female patients (B) after exclusion of one long-surviving patient (shown in A).

<p>Correlation between HDL triglycerides and time of survival in female patients (A); HDL triglycerides and time of survival in female patients (B) after exclusion of one long-surviving patient (shown in A).</p

Serum concentrations of oxysterols conjugated with sulphuric acid in humans.

a<p>free+esterified+sulphated, m - months.</p

Serum concentrations of oxysterols conjugated with sulphuric acid in mice.

a<p>free+esterified+sulphated.</p

Recovery Experiments using pooled serum sample of healthy volunteers.

a<p>24S-hydroxycholesterol,</p>b<p>25-hydroxycholesterol,</p>c<p>Measured after solvolysis with sulphate added,</p>d<p>cholesterol-sulphate,</p>e<p>24OHC-sulphate,</p>f<p>25OHC-sulphate.</p